Bioinformatics analysis of laccases involved in active lignans′ biosynthesis in Isatis indigotica
10.3969/j.issn.1006-0111.2017.03.005
- VernacularTitle:催化菘蓝活性木脂素生物合成的漆酶基因家族生物信息学分析
- Author:
Liang CHEN
1
;
Qing LI
;
Junfeng CHEN
;
Jingxian FENG
;
Lei ZHANG
;
Wansheng CHEN
Author Information
1. 福建中医药大学药学院
- Keywords:
Isatis indigotica Fort;
laccase;
bioinformatics;
lariciresinol
- From:
Journal of Pharmaceutical Practice
2017;35(3):208-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective Identifying laccases, as one of the key synthetases in the lariciresinol biosynthetic pathway, by analyzing the transcriptome sequencing results in Isatis indigotica would provide a dependable foundation for later functional study of Isatis indigotica′s laccases. Methods Bioinformatical softwares and kinds of analytical methods online were used to find out the characteristics of the laccases from I. indigotica, including physical and chemical properties, homology, and the properties after induction of MeJA. Results The transcriptional results showed that Iilac3 and Iilac5 from I. indigotica were corresponded ith the accumulation of the effective metabolites, making them the potential functional genes participated in lariciresinol synthesis. Conclusion Through the detailed bioinformatical analysis of Iilacs,which laid a solid foundation for the further study of the physiological and biochemical mechanisms and structural characteristics of the functional proteins.
