Simultaneous determination of 14 amino acids in Pfaf fia by RP-HPLC with pre-column derivatization
10.3969/j.issn.1006-0111.2017.02.008
- VernacularTitle:柱前衍生RP-HPLC法同时测定珐菲亚中14种水解氨基酸
- Author:
Zhe CHEN
1
;
Juan LU
;
Jiming PENG
;
Ruiping CHAI
;
Xi CHEN
Author Information
1. 中国医学科学院药用植物研究所
- Keywords:
Pfaf fia;
pre-column derivatization;
RP-HPLC;
amino acids;
phenyl isothiocyanate
- From:
Journal of Pharmaceutical Practice
2017;35(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for determining 14 hydrolyzed amino acids in Pfaf fia .Methods The sample was derivatized with phenyl isothiocyanate (PITC) .Amino acids were separated on Waters XBridge Shield RP18 (4.6 mm × 250 mm ,5 μm) column at the flow rate of 0.8 ml/min ,detected at 254 nm .The column temperature was 25 ℃ . Results The response was linear for 14 amino acids with a correlation coefficient r>0 .9990 .The average recoveries (n=6) were 90 .2%-105 .1% .Amino acids derivative solution remained stable in 24 hours .Conclusion This well-established method is very reliable .It can be used as a quantitative determination method for 14 amino acids in Pfaf fia .
