Determination of baicalin in Biyanling capsule by HPLC
10.3969/j.issn.1006-0111.2017.01.015
- VernacularTitle:高效液相色谱法测定鼻炎灵胶囊中黄芩苷含量
- Author:
Liangjun GUO
1
;
Liang SUN
;
Wei ZHENG
;
Ping LU
;
Mingqiang FU
;
Jianbin GUO
Author Information
1. 解放军98医院
- Keywords:
HPLC;
Biyanling capsule;
baicalin;
content determination
- From:
Journal of Pharmaceutical Practice
2017;35(1):64-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for determination of baicalin .Methods The separation was carried out on a Waters XBridge C18 column(4 .6 mm × 250 mm ,5μm) ,the mobile phase was composed of acetonitrile and 0 .8% for-mic acid (25∶75) ,the detection wavelength was set at 276 nm ,the flow rate was 1 .0 ml/min ,the column temperature was 30 ℃ and the injection volume was 10 μl .Results The linearity was obtained over 1 .25-40 μg/ml(r=0 .999 9) for baicalin . The RSD of precision were less than 2% .The average recovery was between 95% and 100% .Conclusion This HPLC method was simple ,accuracy and suitable for the quality control of Biyanling capsule .
