Paclitaxel loaded nanoparticles with cholesterol succinyl bletilla striata polysac-charide as a carrier
10.3969/j.issn.1006-0111.2017.01.012
- VernacularTitle:用疏水改性的白及多糖制备载紫杉醇纳米粒并对其表征
- Author:
Nian CHENG
1
;
Wencui ZHAO
;
Qi ZHANG
;
Yanping WANG
;
Dan HAN
;
Xuanang XIAO
Author Information
1. 延边大学药学院
- Keywords:
bletilla striata polysaccharide;
hydrophobic modification;
Paclitaxel;
nanoparticles;
antitumor
- From:
Journal of Pharmaceutical Practice
2017;35(1):48-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of bletilla striata polysaccharide as a drug delivery material .Method With hydrophobic cholesterol succinyl bletilla striata polysaccharide (CHSB) as the carrier ,the model drug paclitaxel (PTX) loaded nanoparticles were prepared via dialysis method .The morphology of nanoparticles was observed by Transmission Elec-tron Microscope (TEM) .The particle size ,distribution and zeta potential were characterized by Dynamic Light Scattering in-strument (DLS) .The entrapment efficiency ,drug loading and in vitro release were determined by high performance liquid chromatography (HPLC) .Differential Scanning Calorimetry (DSC) was used to confirm the drug form in the drug loaded nan-oparticles .The in vitro antitumor activity of nanoparticles was assayed by MTT .The uptake of nanoparticles by QGY-7703 liver cancer cells was observed by fluorescence labeling method .Results The prepared nanoparticles had spherical shape with uniform particle size distribution .The drug was loaded in the interior of the nanoparticles .Drug loading and encapsulation effi-ciency were affected by the CHSB in certain range .The cytotoxicity of drug loaded nanoparticles on liver cancer cells was stron-ger than the free drug .The fluorescence of Rhodamine B labeled drug nanoparticles were observed in the cells .Conclusion Cholesterol succinyl bletilla striata polysaccharide (CHSB) is highly feasible as an insoluble drug carrier .It can be used as a po-tential Nano carrier material .
