Simultaneous determination of oleanolic acid and ursolic acid in Hawthorn extract by HPLC
10.3969/j.issn.1006-0111.2016.06.014
- VernacularTitle:HPLC法同时测定山楂提取物中齐墩果酸和熊果酸的含量
- Author:
Ling MAO
1
;
Guobing WEI
;
Ji YE
;
Bailun ZHOU
;
Yinan LIN
Author Information
1. 江西中医药大学药学院
- Keywords:
Hawthorn extract;
oleanolic acid;
ursolic acid;
quality control;
HPLC
- From:
Journal of Pharmaceutical Practice
2016;34(6):537-539,555
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for simultaneous determination of oleanolic acid and ursolic acid and provide the basis for quality control of Hawthorn extract .Methods Chromatographic separation was performed on Agilent Zorbax SB C18 column(250 mm × 4 .6 mm ,5 μm) with mobile phase of methanol (A)-0 .06 mol/L ammonium acetate solution (B) (85∶15 ,V/V ) under isocratic elution for 30 min .The flow rate was set at 0 .8 ml/min and the detection was set at the wavelength of 210 nm .Results Oleanolic acid and ursolic acid showed good linearity (r> 0 .999 5) in the ranges of 0 .496-2.480 g and 0 .498-9 .960 g ,respectively .Repeatability ,precision ,recovery and stability were conform to the method valida-tion requirements of China Pharmacopoeia .Conclusion The method could provide the basis for the quality control of Hawthorn extract and its preparation .
