Study on quality standards of hospital preparation Ganmao granules

VernacularTitle:医院制剂感冒颗粒质量标准研究
Author: Weihua JIN ¹ ; Botao YU ; Hua CHEN ; Ming ZHANG ; Kaihua FAN ; Zhiqiang PU ; Zonghui SONG
Author Information

1. 原成都军区总医院药剂科
Keywords: Ganmao granules; baicalin; TLC; HPLC
From: Journal of Pharmaceutical Practice 2016;34(6):534-536,551
CountryChina
Language:Chinese
Abstract: Objective To establish a quality standard for Ganmao granules of hospital preparations .Methods Radix Scutellariae ,cortex phellodendri and radix bupleuri were identified by thin layer chromatography (TLC) qualitatively .High performance liquid chromatography (HPLC) was used for the content of baicalin .The determination was performed on Agilent HC-C18 column (250 mm × 4 .6 mm ,5 μm) at 30 ℃ with mobile phase composed of methanol-0 .2% phosphoric acid (43∶57) at the flow rate of 1 .0 ml/min .The detection wavelength was set at 280 nm .Results In TLC chromatograms ,the spectra of different test products had spots of the same color at corresponding sites ,with no interference from negative control .A good linearity range of baicalin was 1 .81~72 .40 μg/ml (r=0 .999 9) .The average recovery rate was 98 .55% (RSD=1 .91% ,n=9) .Conclusion The quality standard was established and the method of identification has good reproducibility .The method of determination of baicalin content improved the controllability of formulated quality of Ganmao granules .