Study on quality control of Jianpibushen granules
10.3969/j.issn.1006-0111.2016.06.012
- VernacularTitle:健脾补肾颗粒的质量标准研究
- Author:
Shaolan XU
1
;
Dan ZHAO
;
Junqin MAO
Author Information
1. 解放军85医院
- Keywords:
Jianpibushen granules;
quality standard;
peoniflorin;
TLC;
HPLC
- From:
Journal of Pharmaceutical Practice
2016;34(6):530-533
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quality standard for Jianpibushen granules .Methods The five main ingredients of the formulation :Astragalus membranaceus ,Salvia miltiorrhiza Bunge ,Codonopsis pilosula ,Citrus reticulata Blanco and Paeonia lactif lora Pall ,were identified by thin layer chromatography (TLC) respectively .The content of peoniflorin in Paeo-nia lactif lora was determined by high performance liquid chromatography (HPLC) .The separation was performed on Agilent Eclipse Plus C18 column(4 .6 mm × 250 mm ,5 μm) .The mobile phase was acetonitrile and 0 .1% potassium dihydrogen phos-phate solution for gradient elution .The flow rate was 1 ml/min ,the column temperature was 25 ℃ ,the UV detection was per-formed at 230 nm .Results The spots in TLC were clear without interference in negative control .A good linear relationship was shown within the range of 8 .676-277 .632 μg /ml for peoniflorin (r=0 .999 9) .Conclusion This method is simple ,accu-rate and reproducible ,and can be used as an effective quality control of Jianpibushen granules .
