Determination of baicalin in Kangdeling capsule by HPLC

VernacularTitle:高效液相色谱法测定康得灵胶囊中黄芩苷的含量
Author: Shuwen JIANG ¹ ; Jieping LIU ; Qiusheng XIAO ; Jinbao XIAO ; Chunshan SHI ; Dan SUN ; Zongwen JIANG
Author Information

1. 解放军208医院461临床部
Keywords: baicalin; HPLC
From: Journal of Pharmaceutical Practice 2016;34(5):447-449
CountryChina
Language:Chinese
Abstract: Objective To establish an HPLC quantitative method for the content determination of baicalin in Kangdeling capsule .Methods The chromatography column was Agilent Tc-C18-WR (4 .6 mm × 250 mm ,5μm) ,and the column temper-ature was 30 ℃ .The mobile phase consisted of acetonitrile and 0 .5‰ phosphoric acid (26 ∶ 74 ) .The flow rate was 1 .0 ml/min ,and the detection wavelength was 265 nm .Results The retention time of baicalin was about 16 min .The calibra-tion equation was Y=22 114 .67 X -112 836 .7(r=0998 8)with good linearity in the range of 5 .410-108 .2 μg/ml for baicalin . The average recovery was 98 .78% while RSD were 0 .74% .Conclusion This method is simple ,time-saving and accurate which could be used to routine analysis of baicalin in Kangdeling capsule .