Determination of matrine in Lefu oral liquid by HPLC
10.3969/j.issn.1006-0111.2016.05.016
- VernacularTitle:高效液相色谱法测定乐肤口服液中苦参碱的含量
- Author:
Xianglei WU
1
;
Ming YUE
;
Hao HUANG
;
Wenquan LU
;
Shujuan PIAO
;
Feng ZHANG
Author Information
1. 上海长征医院药学部
- Keywords:
HPLC;
matrine;
Lefu oral liquid
- From:
Journal of Pharmaceutical Practice
2016;34(5):443-446
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chromatography method for the determination of matrine in Lefu oral liquid .Methods The HPLC method was performed on a Diamonsil Platisil NH2 column (4 .6 mm × 250 mm ,5μm) with the mobile phase of acetonitrile-isopropyl alcohol-3% phosphoric acid solution (84 ∶ 4 ∶ 12 ) . The flow rate was 1 .2 ml/min .The sample injection volume was 5 μl .The detective wavelength was 205 nm .Results The calibration curve of matrine showed good linear response ranged from 54 .50 to 872 .00 μg/ml with r=0 .999 1 .The average recovery of spiked samples for matrine was 99 .82% while the relative standard deviation for repetitions was 1 .12% .Conclusion The method was simple ,reliable and repeatable ,which could be used for the quantitative determination of matrine of Lefu oral liquid .
