TLC identification and determination of total flavonoids in Compound caulis Polygoni multiflori mixture
10.3969/j.issn.1006-0111.2016.05.011
- VernacularTitle:复方首乌藤合剂的薄层鉴别和总黄酮的含量测定
- Author:
Qiping ZENG
1
;
Yixiang CAO
;
Lishan HUANG
;
Rong ZHANG
Author Information
1. 解放军175医院制剂科
- Keywords:
Compound caulis Polygoni multiflori mixture;
TLC;
total flavonoids;
ultraviolet spectrophometry;
content
- From:
Journal of Pharmaceutical Practice
2016;34(5):424-427
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quality control standards for Compound caulis Polygoni multiflori mixture .Meth-ods Caulis polygoni multiflori ,cortex alibiziae and schisand chinensis were identified by TLC ,total flavonoids were deter-mined by UV .Results Caulis polygoni multiflori ,cortex alibiziae and schisand chinensis could be detected by TLC .A better linear relationship between concentration and absorbance in the range of 9 .12~27 .36 μg/ml .The linear regression equation was A=0 .034 55 C-0 .110 34 (r=0 .999 2) ,The average recovery of emodin was 101 .0% (RSD=1 .58% ) .Conclusion The method is simple ,quick ,accurate and sensitive ,which could be used as a quantitative analysis method for this preparation .
