Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang gran-ules by HPLC-ELSD

VernacularTitle:HPLC-ELSD法同时测定小儿宝泰康颗粒中贝母素甲与贝母素乙的含量
Author: Liru SHEN ¹ ; Ruihong LI ; Tailiang WANG
Author Information

1. 唐山市食品药品检验中心
Keywords: Xiaoer Baotaikang granules; peimine; peiminine; HPLC-ELSD
From: Journal of Pharmaceutical Practice 2016;34(4):351-353
CountryChina
Language:Chinese
Abstract: Objective To develop a new high performance liquid chromatography (HPLC ) coupled with Evaporative Light Scattering Detector (ELSD) method for simultaneous determination of two indicative components (peimine and peimi-nine) in Xiaoer Baotaikang granules .Methods The analysis was performed on a Thermo C18 column (250 mm × 4 .6 mm , 5 μm) by using a mobile phase consisted of acetonitrile-water and diethylamine (65 ∶ 35 ∶ 0 .03) ,and the flow rate was 1 .0 ml/min;ELSD parameters :the temperature of drift tube was 85 ℃ and the carrier gas flow rate was 2 .2 L/min .Results The linear ranges of peimine and peiminine were 0 .586~5 .860 μg (r=0 .999 6) and 0 .564~5 .640 μg (r=0 .999 9) ,respec-tively .The average recovery rate for peimine and peiminine was 99 .44% (RSD was 0 .7% ) and 98 .56% (RSD was 1 .0% ) , respectively .Conclusions The method is sample ,accurate and reproducible ,which could be used for the quality control of Xiao-er Bao taikang g ranules .