Study on the rat pulmonary i rritation of aerosol inhaled Tanreqing and Reduning
10.3969/j.issn.1006-0111.2016.04.016
- VernacularTitle:雾化吸入痰热清、喜炎平对大鼠肺部的刺激性研究
- Author:
Ning TAN
1
;
Pengyue LI
;
Boyu DONG
;
Mengdi ZHAO
;
Liu YANG
;
Yang LU
Author Information
1. 北京中医药大学中药学院
- Keywords:
pulmonary delivery;
Tanreqing;
Reduning;
excurrent bronchoalveolar lavage;
irritation
- From:
Journal of Pharmaceutical Practice
2016;34(4):348-350,356
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the rat pulmonary irritant of aerosol inhaled Tanreqing and Reduning injection .Methods Rats were devided into two groups for each medicine (low concentration group and high concentration group ) ,nebulized drug administration for seven days ,with the control group irrigated with saline ,and were sacrificed .Through bronchoalveolar lav-age ,excurrent bronchoalveolar lavage fluid (BALF) was used for total protein determination and LDH vitality test to evaluate pulmonary toxicity of two medicines .Results The protein concentrations of two groups in low and high concentrations of Tan-reqing and Reduning respectively are (193 .78 ± 27 .74) ,(235.33 ± 50.41)μg/ml;(174 .02 ± 17 .82) ,(227 .27 ± 66 .03)μg/ml;LDH vitalities respectively are 1065 .21 ± 181 .76 ,1467 .33 ± 101 .87;307 .97 ± 47 .56 ,1377 .29 ± 566 .48 .By t-test ,compared with normal saline ,there was no significant effect among these five groups on protein concentration ,but these two medicine were able to improve LDH activity (P<0 .05) which was more obvious in high concentration group .When two medicines were in low concentration ,LDH activity was higher in Tanreqing group with statistical significance (P<0 .05) .Conclusion Aero-sol inhaled Tanreqing and Reduning injection in rats have some pulmonary irritation and potential safety hazard in this delivery w ay .
