Study on quality control of Xinnaoling granules
10.3969/j.issn.1006-0111.2016.04.014
- VernacularTitle:新脑灵颗粒的质量标准研究
- Author:
Xinxin REN
1
;
Xianyou WANG
;
Wenquan LU
;
Shujuan PIAO
;
Shouhong GAO
Author Information
1. 第二军医大学附属长征医院药学部
- Keywords:
Xinnaoling granules;
salvianolic acid B;
HPLC;
TLC
- From:
Journal of Pharmaceutical Practice
2016;34(4):338-342
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish quality control of Xinnaoling granules .Methods Thin layer chromatography (TLC) methods were used to identify Rhizoma Corydalis ,Radix Angelicae dahuricae ,Cortex Magnoliae officinalis ,Radix Glycyrrhi-zae ,Rhizoma Ligusticum ,Ligusticum wallichii ,Schisandra Chinensis ,Dendranthema morifolium .The concentration of salvi-anolic acid B was determined by high performance liquid chromatography (HPLC) .The method employed a column of Agilent Eclipse XDB-C18 (4 .6 mm × 250 mm ,5 μm) with a mobile phase of 0 .5% formic acid (A)-acetonitrile (B) at a temperature 25℃ .The gradient elution program was as follow :0~5 min 24% B ,5~6 min 24% ~19% B ,6~25 min 19~20% B .The flow rate was 1 .0 ml/min ,and the injection volume was 10μl and the detection wavelength was 286 nm .Results The spots in TLC plates were clear and specific .As for salvianolic acid B ,the linear range was 20-1 280 μg/ml and the equation of linear regres-sion of salvianolic acid B was Y=13 .304 X -117 .50 (r=0 .999 9 ,n=7) .The average recovery rate was 96 .17% (RSD=1 . 10% ) .Conclusion The method was proved to be simple ,reliable ,reproducible ,and could be used in the quality control of Xinnaoling g ranules .
