Effect of Electroacupuncture on Visceral Hyperalgesia, Serotonin and Fos Expression in an Animal Model of Irritable Bowel Syndrome.
- Author:
Justin CY WU
1
;
Eric TC ZIEA
;
Lixing LAO
;
Emma FC LAM
;
Catherine SM CHAN
;
Angela YQ LIANG
;
Sunny LH CHU
;
David TW YEW
;
Brian M BERMAN
;
Joseph JY SUNG
Author Information
1. Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong. justinwu@cuhk.edu.hk
- Publication Type:Original Article
- Keywords:
Electroacupuncture;
Hyperalgesia;
Brain-gut axis;
Fos;
Serotonin
- MeSH:
Acupuncture;
Acupuncture Points;
Animals;
Axis, Cervical Vertebra;
Benzodiazepines;
Brain Stem;
Colon;
Down-Regulation;
Electroacupuncture;
Epithelium;
Horns;
Hyperalgesia;
Immunohistochemistry;
Irritable Bowel Syndrome;
Models, Animal;
Nociceptive Pain;
Pain Threshold;
Raphe Nuclei;
Rats;
Rats, Sprague-Dawley;
Reflex;
Salicylamides;
Serotonin;
Spinal Cord;
Visceral Pain
- From:Journal of Neurogastroenterology and Motility
2010;16(3):306-314
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: While it is well established that acupuncture relieves somatic pain, its therapeutic effect on visceral pain such as irritable bowel syndrome (IBS) is unclear. We evaluated the effect of acupuncture in treating visceral hyperalgesia in an animal model. METHODS: Sprague-Dawley rats (n = 8 per group) with prior neonatal maternal separation stress were randomly allocated to receive 3-day treatment of either electroacupuncture (EA) or sham acupuncture at acupoint ST-36. Another group of rats without prior maternal separation was included as non-handled controls. Colorectal distension was performed on the day after acupuncture treatment. The 3 groups were compared for pain threshold as determined by abdominal withdrawal reflex and visceromotor response as measured by electromyogram. Colon, spinal cord, and brainstem were sampled for topographic distribution and quantitative assessment of serotonin and Fos expression by immunohistochemistry. RESULTS: Rats in EA group had significantly higher pain threshold compared to those in sham acpuncture group (25.0 +/- 5.7 mmHg vs 18.7 +/- 5.2 mmHg, p = 0.01) and it was comparable with that of non-handled treatment naive controls (29.4 +/- 9.3 mmHg, p = 0.28). They also had lower visceromotor response as measured by electromyogram compared to those received sham acupuncture at all colorectal distension pressures. EA significantly suppressed Fos expression in doral raphe nuclei of brainstem, superficial dorsal horn of spinal cord and colonic epithelium but suppressed 5-HT expression only in brainstem and spinal cord. CONCLUSIONS: Electro acupuncture attenuates visceral hyperlagesia through down-regulation of central serotonergic activities in the brain-gut axis.
