Quantitation of nine ginsenosides in Panax ginseng hairy roots and other Panax ginseng plant components by HPLC
10.3969/j.issn.1006-0111.2016.04.007
- VernacularTitle:HPLC法测定人参毛状根及不同人参样品中9种人参皂苷的含量
- Author:
Ling CHEN
1
;
Chunyan LI
;
Zheng WANG
;
Min JIA
;
Ting HAN
Author Information
1. 第二军医大学药学院生药学教研室
- Keywords:
Panax ginseng decoction pieces;
hairy root;
Panax ginseng;
ginsenoside;
HPLC
- From:
Journal of Pharmaceutical Practice
2016;34(4):313-317,353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the quantitation of nine ginsenosides (Rc ,Rb1 ,Rb2 ,Re ,Rd , Rg1 ,Rg2 ,Rg3 and Rh2 ) in Panax ginseng samples .Methods An HPLC method was developed to determine the quantities of the nine ginsenosides .The determination was performed on a Zorbax SB C18 column (4 .6 mm × 250 mm ,5μm) with an Extend-C18 guard column (4 .6 mm × 12 .5 mm ,5 μm) at 35 ℃ .The mobile phase was a multi-step acetonitrile-water gradient run at a flow rate of 1 .0 ml/min .The detection wavelength was 203 nm .Results The nine ginsenosides were baseline separated with-in 120 min .The method had good linearity ,precision ,stability and reproducibility with RSDs all less than 2 .0% .The sample recoveries were between 98 .3% to 102% .The quantity of total saponins in leaves and fibrous roots of Panax ginseng ,which were measured as 48 .9 mg/g and 23 .6 mg/g ,respectively ,were higher than those in the other plant components .The amounts of total saponins in Panax ginseng hairy roots were similar to those in taproots and fruits of Panax ginseng ,which was 7 .47 mg/g .Conclusion The established HPLC method is accurate ,simple ,rapid ,precise and reproducible and could be used for the quantitation of these nine ginsenosides in Panax ginseng samples .
