Determination of valproate in serum by precolumn derivatization HPLC

VernacularTitle:反相高效液相色谱-柱前衍生法测定人血清中丙戊酸浓度
Author: Bi CHEN ¹ ; Xiaoliang HUANG ; Hui LI
Author Information

1. 湖南省郴州市第一医院药学部
Keywords: valproate; serum concentration; derivatization; HPLC
From: Journal of Pharmaceutical Practice 2016;34(3):255-257
CountryChina
Language:Chinese
Abstract: Objective To establish a determination method for valprate in serum .Methods Serum sample was acidified by sulfuric acid and extracted with ethyl ether ,cyclohexanecarboxylic acid was selected as an internal standard ,α-bromoaceto-phenone as derivative reagent .Determination was performed with HPLC with methanol :water (70:30) as the mobile phase . The nalytical column was Eclipse Plus C18 (150 mm × 4 .6 mm ,5 μm) ,detected at 248 nm ,the flow rate was 1 .0 ml/min .Re-sults The linear rang of valproate was 8 .65~173 μg/ml .The mean relative recovery was bigger than 99 .27% .Both the rela-tive standard deviation (RSD) of inter-day and intra-day was less than 5% .Conclusions The method is rapid ,accurate ,sensi-tive and suitable for clinical therapeutic drug monitoring .