Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra

VernacularTitle:红参高效液相色谱指纹图谱研究
Author: Yanxia GAO ¹ ; Juan SU ; Huizi JIN
Author Information

1. 上海交通大学药学院
Keywords: Ginseng Radix et Rhizoma Rubra; HPLC; fingerprint
From: Journal of Pharmaceutical Practice 2016;34(3):249-251,254
CountryChina
Language:Chinese
Abstract: Objective To establish the HPLC fingerprint of Ginseng Radix et Rhizoma Rubra for its quality control . Methods Sharpsil-T C18 column(4 .6 mm × 250 mm ,5 μm)was used with acetonitrile-water in gradient elution mode .The flow rate was 1 .0 ml/min ,the column temperature was maintained at 30 ℃ ,and the detective wavelength was 203 nm .Results 24 co-possessing peaks were selected as fingerprint peaks and the similarities between each of the ten areas and the standard chro-matographic fingerprints of Panax ginseng Rubra were calculated while Rb1 peak selected as the reference peak .The similarity was more than 0 .940 .Eleven chemical compounds were identified by comparing the reference substance .Conclusion The method could be used for the quality control of Panax ginseng Rubra with good precision ,stability ,and reproducibility .