The fingerprint and durability study on various regions of Polygonum cuspidatum
10.3969/j.issn.1006-0111.2016.02.019
- VernacularTitle:虎杖饮片指纹图谱的研究及其耐用性考察
- Author:
Hongwei LIN
1
;
Shujuan PIAO
;
Chunxia JIANG
;
Jingxian LI
;
Wenquan LU
;
Wansheng CHEN
Author Information
1. 第二军医大学附属长征医院药学部
- Keywords:
Polygonum cuspidatum;
Chinese herbal medicine;
HPLC;
fingerprint;
durability
- From:
Journal of Pharmaceutical Practice
2016;34(2):174-176,187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the fingerprint of Polygonum cuspidatum from various regions by HPLC and provide technical support for the identification.Methods HPLC conditions :the chromatographic column was Agilent ZORBAX SB-C18 (4.6 mm × 250 mm,5 μm);the mobile phase was 0.1% formic acid in water and acetonitrile (A-B);gradient elution ;the de-tection wavelength was 306 nm ;the flow rate was 1 ml/min;the column temperature was 30 ℃ ;injection volume was 20 μl. Results The HPLC gradient conditions could effectively separate polydatin,resveratrol,emodin and emodin ether,etc.Con-clusion This method is stable,effective and durable,which is instructive to the herbs data identification of Polygonum cuspi-datum.
