Assay of eupatilin and arteanoflavone in Artemisia anomala by HPLC
10.3969/j.issn.1006-0111.2016.02.016
- VernacularTitle:应用高效液相色谱法测定中药奇蒿中异泽兰黄素和奇蒿黄酮的含量
- Author:
Zhiquan QIU
1
;
Qingqing CAO
;
Weifeng TAN
;
Jin ZHOU
;
Lei LÜ
Author Information
1. 第二军医大学附属东方肝胆外科医院 胆道一科
- Keywords:
Artemisia anomala;
eupatilin;
arteanoflavone;
HPLC;
determination
- From:
Journal of Pharmaceutical Practice
2016;34(2):163-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the concentration of eupatilin and arteanoflavone in A rtemisia anomala by high per-formance liquid chromatography (HPLC).Methods A rtemisia anomala was extracted by ultrasonic for 60 minutes with 10 times volume of methanol.The HPLC was performed on a SHISEIDO MG-C18 column (3.0 mm × 100 mm,3μm).The mobile phase was a mixture of acetonitrile (ACN) and 0.1% formic acid (40:60,V/V ).The detection wavelength was 350 nm,the column temperature was 25 ℃ and the injection volumn was 5μl.Results Eupatilin and arteanoflavone were separated at base-line within 15min with good linearity.The method validation results show that the precisions,repeatability and stability were all in the normal range.The low,medium and high level recoveries of eupatilin were 100.26%,99.58%,102.24%,and those of arteanoflavone were 99.09%,101.12%,101.43%,respectively.Conclusion The method was rapid,simple,reproductive and accurate.It can be used to control the quality of Artemisia anomala.
