Comparision two kinds of content determination method of mannatide for injec-tion
10.3969/j.issn.1006-0111.2016.01.017
- VernacularTitle:注射用甘露聚糖肽两种含量测定方法的比较
- Author:
Weihua JIN
1
;
Hua CHEN
;
Ming ZHANG
;
Zhiqiang PU
Author Information
1. 成都军区总医院药剂科
- Keywords:
mannatide for injection;
HPLC;
spectrophotometry;
D-mannose
- From:
Journal of Pharmaceutical Practice
2016;(1):62-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare two methods in the determination of mannatide for injection .Methods The contents were determined by spectrophotometry and HPLC method .Results In the samples determined by Spectrophotometric Determi‐nation ,soluble in phenol solution and sulfuric acid ,was detected at wavelength 490 nm ,D‐mannose derivatives linear concen‐tration range is 10 .2‐51 μg/ml (r=0 .999 1) ,the average content was 92 .14% ,the average RSD value was 1 .17% .In the HPLC determination , with color spectrum column : Gemini C18 (250 mm × 4 .6 mm , 5 μm ) , mobile phase acetonitrile -0 .02 mol/L ammonium acetate solution (20∶80) ,flow rate:1 ml/min ,the detection wavelength was at 250 nm , column temperature:30 ℃ ,sample size:10 μl detection .The average content ws 83 .47% ,the average RSD value was 0 .65% ,the content of two kinds of methods could effectively determine the D‐mannose .Conclusion The average content for spectrophotometry was higher ,but the phenol was used in detection of special odor ,poisonous ,corrosive ,would cause a cer‐tain risk .HPLC was exclusive ,chromatogram was more intuitive ,on the operator harmfulness and less irritating .A new meth‐od for the determination of D‐mannose was established at the same time .
