Study on pharmacokinetics and determination of actarit in human plasma by HPLC

VernacularTitle:高效液相色谱法测定人血浆中阿克他利的浓度及其药动学研究
Author: Juan HE ¹ ; Chengjie ZHOU
Author Information

1. 上海中医药大学附属曙光医院药剂科
Keywords: actarit; HPLC; pharmacokinetics
From: Journal of Pharmaceutical Practice 2016;(1):41-43,75
CountryChina
Language:Chinese
Abstract: Objective To develop a simple and sensitive HPLC‐UV method to determined actarit in plasma .Methods The chromatographic separation was carried out on a Dikma C18 column with a mobile phase composed of water containing 1.2% acetic acid‐methanol (50∶50 ,V/V ) at a flow rate of 1 .0 ml/min and the temperature of column was 30℃ .UV wave‐length mode was monitored at 245 nm .Results A linear response function was established for the range of concentrations 75‐4 000 ng/ml for actarit .The inter‐and intra‐day precision value were less than 10% for actarit and the accuracy was more than 90% .The extraction recovery was more than 88% .Conclusion This simple ,sensitive ,and exclusive method could be used in routine clinical practice to monitor actarit concentrations in human plasma .