Determination of the content of baicalin in Qiguanyan granules by HPLC
10.3969/j.issn.1006-0111.2015.05.019
- VernacularTitle:高效液相色谱法测定气管炎颗粒中黄芩苷的含量
- Author:
Binguo LIU
1
;
Xinping ZHANG
Author Information
1. 解放军254医院
- Keywords:
Qiguanyan granules;
baicalin;
HPLC
- From:
Journal of Pharmaceutical Practice
2015;(5):451-452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of HPLC for the determination of baicalin in Qiguanyan granules .Methods HPLC was performed on ZORBAX SB-C18 (4 .6 mm × 150 mm ,5 μm) column with mobile phase consisted of methanol-0.1%phosphoric acid (47∶ 53) at ambient temperature .The flow rate was 1 .0 ml/min .The detection wavelength was 280 nm . Results Baicalin showed a good linearity in the range of 6 .63-210 μg/ml(r=0.999 8 ,n= 6) ,and the average recovery was 98.16% (RSD=1.89% ) .Conclusion This methods was simple ,accurate ,sensitive ,reproducible and reliable ,which was suit-able for the content control of Qiguanyan granules .
