Simultaneous determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing oral solution by HPLC
10.3969/j.issn.1006-0111.2015.05.017
- VernacularTitle:高效液相色谱法测定麻杏口服液中盐酸麻黄碱及盐酸伪麻黄碱的含量
- Author:
Minxin ZHANG
1
;
Aiwen HUANG
;
Hongtao SONG
Author Information
1. 南京军区福州总医院药学科
- Keywords:
Maxing oral solution;
ephedrine hydrochloride;
pseudoephedrine hydrochloride;
HPLC
- From:
Journal of Pharmaceutical Practice
2015;(5):445-447
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing oral solution .Methods Phenomenex Hydro-RP (250 mm × 4 .6 mm ,4 μm) was adopted .Acetonit-nile (A) and 0 .1% phosphonic acid solution (0 .1% triethanolamine solution)(B) was used as gradient mobile phase(0-20 min , 3% →10% A)at flow rate was 1 .0 ml/min and the program of UV gradient absorbance detection was 210 nm .The sample vol-ume was 20 μl .Results A good linearity was obtained over the concentration range of 0 .99-39 .6 μg/ml for ephedrine hydro-chloride (r=0 .999 9) and 1 .09-43 .6 μg/ml for pseudoephedrine hydrochloride (r=0 .999 9) .The average recovery of ephed-rine hydrochloride was 101 .5% with RSD of 1 .77% (n=6) ,and the average recovery of pseudoephedrine hydrochloride was 100 .8% with RSD of 1 .96% (n=6) .Conclusion This method was simple ,accurate and quick ,which could be used for deter-mination and quality control of Maxing oral solution with good selectivity and repeatability .
