Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xin-baoshen tablets by HPLC
10.3969/j.issn.1006-0111.2015.05.015
- VernacularTitle:高效液相色谱法测定新保肾片中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量
- Author:
Liye QIAO
1
;
Li FANG
;
Yanli CAO
;
Yin LU
;
Hua SU
;
Shudong WANG
Author Information
1. 南京军区南京总医院制剂科
- Keywords:
aloe-emodin;
rhein;
emodin;
chrysophanol;
physcion;
HPLC;
content determination
- From:
Journal of Pharmaceutical Practice
2015;(5):438-440
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish method of the determination of aloe-emodin ,rhein ,emodin ,chrysophanol and physcion in Xinbaoshen tablet ,for the production of quality protection .Methods HPLC was performed on a Lichrospher-C18 column (250 mm × 4 .6mm ,5 μm)with the mobile phase of methanol-0 .1% H3 PO4 (70:30) .The flow rate was 1 .0 ml/min ,the col-umn temperature was at 35 ℃ and the detection wavelength was 254 nm .Results The linear rang of aloe-emodin ,rhein ,emod-in ,chrysophanol and physcion were obtained between 2 .30-18 .4 μg/ml ( r= 1 .0) ,2 .930-23 .44 μg/ml( r= 1 .0) ,5 .00-40 .0 μg/ml ( r=1 .0) ,14 .870-118 .96μg/ml( r= 0 .9998 ) and 7 .410-59 .28 μg/ml(r=0 .999 9) ,the average recoveries were 100 .16% ,102 .91% ,99 .76% ,100 .32% ,100 .44% ,RSD were 1 .58% ,1 .27% ,1 .67% ,1 .33% ,1 .03% (n=9) .Conclusion The improved method was accurate and feasible which could be used convenient in quality control .
