Combination of wogonin with 5-fluorouracil exhibits antagonistic effect on human Hep-G2 hepatocellular carcinoma cells
10.3969/j.issn.1006-0111.2015.05.007
- VernacularTitle:汉黄芩素与氟尿嘧啶联用抗人肝癌细胞He p-G2作用的拮抗效应研究
- Author:
Weizhong CHEN
1
;
Jingbin CAI
;
Xiaolan CHEN
;
Ruiqi CHEN
;
Xiajing LI
;
Lianming LIAO
Author Information
1. 南京军区福州总医院第一附属医院
- Keywords:
wogonin;
fluorouracil;
antagonism;
hepatocellular carcinoma cell Hep-G2
- From:
Journal of Pharmaceutical Practice
2015;(5):411-414
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antagonistic effect of wogonin in combination with 5-fluorouracil (5-FU) on the proliferation of human Hep-G2 hepatocellular carcinoma cells .Methods Human hepatocellular Hep-G2 cells were divided into experimental group (wogonin group ,5-FU group ,wogonin + 5-FU group) and control group .MTT method was used to eval-uate tumor cell proliferation in vitro ,flow cytometry analysis was used to evaluate tumor cell apoptosis .Results The results showed that the wogonin inhibited the proliferation of tumor cells at the concentrations of 5 ,10 ,20 and 40 μmol/L after 24 h and 48 h treatment respectively (P<0.05);5-FU also inhibited the proliferation of tumor cells at the concentrations of 5 ,10 , 20 and 40 mg/L after 24 h and 48 h treatment respectively (P< 0.05) .However when wogonin was combined with 5-FU (wogonin+5-FU group) ,an antagonistic effect was observed on tumor cell proliferation (P<0.05) .When cells were treated by wogonin+5-FU for 48 h ,the combined index (CI) value slowed a dose-dependent antagonistic effect (P<0.05) .Conclusion Wogonin has anti-tumor effect .However when wogonin was combined with 5-FU ,an obvious antagonistic effect on 5-FU′s anti-tumor action was observed .The underlying mechanism deserves further study .
