Immunohistochemical Expression of Stem Cell Markers during the Wound Healing Process of Cutaneous Burn.
- Author:
Young Hee CHOI
1
;
Min Gyu KIM
;
Dong Hyun AHN
;
Seong Jin CHO
;
Soo Hee HONG
;
Jae Yong LEE
;
Jin Won LEE
;
Hae Sung KIM
Author Information
- Publication Type:Original Article
- Keywords: Hairless mouse; Burn; Wound healing; Stem cell
- MeSH: Animals; Bandages, Hydrocolloid; Burns; Cell Movement; Endothelial Cells; Epidermis; Extracellular Matrix; Hair; Hot Temperature; Intermediate Filament Proteins; Keratin-15; Keratins; Mice; Mice, Hairless; Nerve Tissue Proteins; Regeneration; Skin; Stem Cells; Stromal Cells; Water; Wound Healing
- From:Journal of the Korean Surgical Society 2010;79(1):1-7
- CountryRepublic of Korea
- Language:Korean
- Abstract: PURPOSE: A cutaneous wound healing requires a well-orchestrated integration of the complex biological and molecular events of cell migration and proliferation, extracellular matrix deposition, angiogenesis and remodeling. Finally, skin regeneration is the main goal. Stem cells are self-renewing multipotent progenitors with the broadest developmental potential in a given tissue at a given time. The aim of this study was to examine the role of stem cells during the wound healing process of cutaneous burn in hairless mice by using immunohistochemical stainings (nestin, cytokeratin 15 and CD31). METHODS: Each mouse received 2 burns at the dorsal area by applying a metal stick heated in boiling water. Burn wound sites were dressed with duoderm. The mice were sacrificed at 0, 2, 7, 14 and 21 days after burn. Histological findings and immunohistochemical expression for stem cell markers were observed. RESULTS: Nestin was expressed in the stromal cells beneath the epidermis, hair follices, dermal cysts and endothelial cells. Cytokeratin 15 was expressed in the epidermis except in basal cells. On 7 and 14 days after burn, the regenerated epidermis didn't express cytokeratin 15. CD31 was expressed in the endothelial cells on 7 and 14 days after burn. The amount of nestin expression was the highest. CONCLUSION: Our results showed that nestin may have various effects on burn wound healing. Cytokeratin 15 was expressed before burn and after burn. It is likely that other cytokeratin may stimulate epithelial regeneration. CD31 may act in vascular regeneration during burn healing.
