The research of separation and purification technology for effective parts f rom Zhizichi decoction by macroporous absorption resina
10.3969/j.issn.1006-0111.2015.03.012
- VernacularTitle:大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究
- Author:
Yan HAN
1
;
Jun WEN
;
Tingting ZHOU
;
Guorong FAN
Author Information
1. 福建中医药大学药学院
- Keywords:
Zhizichi decoction;
macroporous absorption resin;
separation and purification;
total iri doid glycosides;
total isoflavones
- From:
Journal of Pharmaceutical Practice
2015;(3):238-241
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina . Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry .Tak-ing the content of total iridoid glycosides and total isoflavones as indexes ,the effect of the concentration of sample solution , medicinal herbs on the amount of sample ,concentration and volume of eluent was investigated by single factor test .Results The optimum separation and purification technology was as following :the concentration of sample solution was 0 .1 g/ml ,the volume ratio of resin to material drug was 2∶1 ,the adsorption time was 2 h ,and the sample was firstly eluted with 1 BV wa-ter ,then 6 BV 20% ethanol and 60% ethanol ,and the eluent was collected .Conclusion This optimized separation and purifi-cation technology was reasonable and stable ,and it could be extended to large-scale production applications .
