Comparative analysis of different states of polarized BV 2 cells by GC-MS com-bined with metabonomic technology

VernacularTitle:气相色谱唱质谱联用结合代谢组学方法研究不同极化状态下小胶质细胞的代谢差异
Author: Yan WANG ¹ ; Zhongwang YU ; Si CHEN ; Ling LI ; Zhenyu ZHU
Author Information

1. 第二军医大学药学院药物分析学教研室
Keywords: GC-MS; metabonomic; microglia cell; RT-PCR
From: Journal of Pharmaceutical Practice 2015;(3):226-230
CountryChina
Language:Chinese
Abstract: Objective To analyze the different metabolites of the classical activated (M1) ,alternatively activated (M2) and resting BV2 cells by metabolomics method .Methods The mRNAs of several potential biomarkers were determined by real-time PCR analyses to confirm the state of BV2 cells .Static GC-MS combined with metabolomics technology was used to analyze the metabolic changes .Results There were 15 biomarkers identified between the M1 group and the resting group ,and 15 biomarkers were found in the M2 group and the resting group .Conclusion The present study provides an effective way to reveal the mechanism of the polarization of BV 2 cell ,and it might provide a theoretical basis to prevent or treat the neurodegen-erative diseases .