Effect of raw material purity on the degree of substitution of hydrophobically modified Bletilla striata polysaccharide
10.3969/j.issn.1006-0111.2015.03.008
- VernacularTitle:原料纯度对白及多糖疏水改性反应取代度的影响
- Author:
Xuezhu ZHAO
1
;
Yang LIU
;
Yanping WANG
;
Fuqiang LIU
;
Qi ZHANG
;
Dan LIU
;
Nan ZHAO
;
Dan HAN
;
Qinglan ZHAO
Author Information
1. 延边大学药学院
- Keywords:
the purity of raw materials;
cholesteryl succinyl ester;
substitution degree
- From:
Journal of Pharmaceutical Practice
2015;(3):221-225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the degree of substitution of the cholesteryl by purifying cholesteryl succinyl ester (CHS) ,one of the raw materials .Methods CHS was purified by recrystallization ,the pure CHS structure was characterizate by differential scanning calorimetry (DSC) and X ray diffraction (XRD) ,the free cholesterol content of the pure and crude were determinated by high performance liquid chromatography (HPLC) ,the degree of substitution of the cholesteryl was determina-ted before and after the purified by 1 H-NMR .Result CHS was purer after recrystallization ,with property of crystal ,which could significantly improve the degree of substitution of the cholesteryl .Conclusion The method of purification was simple and feasible ,which was the basis of improving hydrophobically modified Bletilla striata polysaccharide′s degree of substitution of the cholesteryl .
