Research on fingerprint analysis of platycodonis liquid extract by UPLC-ELSD
10.3969/j.issn.1006-0111.2015.02.016
- VernacularTitle:用超高效液相色谱蒸发光散射检测桔梗流浸膏指纹图谱的研究
- Author:
Xueyan BI
1
;
Pan HU
;
Shengbo ZHANG
;
Qingbo ZHANG
Author Information
1. 黑龙江省食品药品检验检测所
- Keywords:
Platycodonis;
liquid extract;
UPLC;
ELSD;
fingerprint
- From:
Journal of Pharmaceutical Practice
2015;(2):153-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a stable method for fingerprint analysis of extractum platycodi liquidum to ensure its u‐niformity and stability .Methods UPLC‐ELSD was applied to obtain a chromatogram with 11 well separated peaks ,and the chromatographic condition was as follows:mobile phase was gradient elution by acetonitrile‐water ,column was ACQUITY UPLC BEH C18 (2 .1 × 100 mm ,1 .7 μm) with the column temperature of 30 ℃ and the flow rate of 0 .2 ml/min .Results The chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM .Similar‐ities were all greater than 0 .90 ,which could meet the requirement for chromatographic fingerprint of TCM .Conclusion The stability ,repeatability and precision of the developed method were all validated ,and the chromatographic fingerprint analysis was a rapid and accurate method for the quality evaluation of traditional Chinese medicine .