Extraction process on total falvonoids from Hy pericum sampsonii by orthogonal experiments

VernacularTitle:正交试验优选元宝草中总黄酮的提取工艺
Author: Yingzhen GONG ¹ ; Chengjian ZHENG ; Luping QIN
Author Information

1. 福建中医药大学药学院
Keywords: Hypericum sampsonii; total flavonoids; extracting technology; orthogonal test
From: Journal of Pharmaceutical Practice 2015;(2):134-137
CountryChina
Language:Chinese
Abstract: Objective To optimize the extraction technology of total falvonoids from Hypericum sampsonii .Methods On the basis of single factor test ,solid‐liquid ratio ,ethanol concentration ,extraction time ,extraction temperature and extrac‐tion times on the extraction rate were studied by taking the content of total falvonoids as indexes .Orthogonal test was conduc‐ted to establish the extraction technology of total falvonoids from Hypericum sampsonii .Results The influence degree of fac‐tors on the extraction rate of total falvonoids was in the order of ethanol concentration ,solid‐liquid ratio ,extraction times ,and temperature .The optimum extraction condition was confirmed as follows:ethanol concentration of 70% ,extraction tempera‐ture of 60 ℃ ,and extraction times of 3 × 1 .5 h ,solid to liquid ratio of 1:20 .Under this condition ,the average extraction rate of total falvonoids reached 2 .38% .Conclusion The test was reasonable in design and credible in findings ,and was easily oper‐ated ,which could provide scientific basis for the batch extraction of total flavonoids from Hypericum sampsonii .