Determination of dexamethasone acetate in Kelaweiding liniment by HPLC
10.3969/j.issn.1006-0111.2015.01.014
- VernacularTitle:高效液相色谱法测定克拉维丁搽剂中醋酸地塞米松含量
- Author:
Mingquan CHANG
1
;
Lin CHEN
;
Xiuli HE
;
Fang CHEN
Author Information
1. 湖北医药学院附属太和医院
- Keywords:
Kelaweiding liniment;
dexamethasone acetate;
HPLC
- From:
Journal of Pharmaceutical Practice
2015;(1):55-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quantitative determination method of dexamethasone acetate in Kelaweiding lini‐ment by HPLC .Methods Column:Diamonsil‐C18 (150 mm × 4 .6 mm ,5 μm) ,mobile phase:methanol‐water‐glacialaceticacid (80:20:0 .5) ,flow rate:1 .0 ml/min ,UV detection wavelength:240 nm ,injection volume :20 μl ,column temperature:30 ℃ . Results The standard curve was linear within the range of 2 .00‐64 .00μg/ml ,linear equation was Y=0 .405 2X+0 .804 2(r=0 .999 9) .The average recovery was 99 .3% with RSD 0 .93% (n=9) .Conclusion This method was simple ,stable and could be used to assay dexamethasone acetate in Kelaweiding liniment .
