Determination of calycosin contents in Wujin capsule by HPLC

VernacularTitle:高效液相色谱法测定乌金胶囊中毛蕊异黄酮的含量
Author: Hui WANG ¹ ; Yang LI ; Fang LÜ ; Shuihua LING ; Yufeng HUANG ; Guoqing ZHANG
Author Information

1. 第二军医大学东方肝胆外科医院药材科
Keywords: HPLC; calycosin; determination; Wujin capsule
From: Journal of Pharmaceutical Practice 2015;(1):53-54,57
CountryChina
Language:Chinese
Abstract: Objective To explore method for determination of calycosin contents in Wujin capsule by HPLC .Methods The sample was studied on ZORBA Bonus‐RP (4 .6 mm × 260 mm ,5 μm) column ,the mobile phase consisted of acetonitrile and H2O‐0.1% HCOOH (gradient elution) ,the temperature of column was 25 ℃ ,the UV detection wavelength was set at 260 nm , flow speed was 1 .0 ml/min and injection volume was 10 μl .Results The calycosin calibration curve was at the range of 7 .4‐117 .6 μg/ml (r=1 .000) ,with their regression function being Y=34 .002 X-3 .451 2 .The recovery of calycosin was 95 .24%(RSD=2 .10% ) .6 batches of Wujin capsule calycosin contents were 9 .26‐23 .14μg/g .Conclusion HPLC was an accuracy and simple method for determining the content of calycosin in Wujin capsule .