Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS
10.3969/j.issn.1006-0111.2014.05.012
- VernacularTitle:芎归汤主要化学成分的HPLC-TOF-MS分析
- Author:
Yanan LU
1
;
Guangguo TAN
Author Information
1. 解放军22医院
- Keywords:
Xionggui Decotion;
HPLC-TOF-MS;
chemical constituents
- From:
Journal of Pharmaceutical Practice
2015;(1):49-52,71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze chemical constituents of Xionggui Decotion by rapid‐resolution (high performance) liquid chromatography‐time of flight mass spectrometry (HPLC‐TOF‐MS) .Methods A Shelloseido column(100 mm × 3 .0 mm ,3 μm)was used to separate .The mobile phase consisted of water containing 0 .1% methane acid and acetonitrile was used as gradient elute .The flow rate was 0 .4 ml/min .TOF‐MS was applied for qualitative analysis under positive ion mode . Results Under LC/MS condition ,47 major constituents in Xionggui Decotion were identified by time of flight mass spectrome‐try and structure‐relevant fragment ions .Conclusion A simple and reliable method using HPLC‐TOF‐MS was established to i‐dentify the chemical constituents of Xionggui Decotion .
