The preparation and the cell uptake of polymer vesicles modified with dual ligands

VernacularTitle:双配体修饰聚合物泡囊的制备及细胞摄取
Author: Fangyuan XIE ¹ ; Yuan YU ; Wenqian GENG ; Yanqiang ZHONG
Author Information

1. 第二军医大学药学院药剂学教研室,上海200433
Keywords: brain targeting; polymer vesicles; blood-brain barrier(BBB)
From: Journal of Pharmaceutical Practice 2015;(1):44-48
CountryChina
Language:Chinese
Abstract: Objective To construct an active targeting drug delivery system‐polymer vesicles(PVs), and examined the cellular uptake. Methods Maleimide‐ polyethylene glycol‐poly(lactic‐co‐glycolic acid)(MAL‐PEG‐PLGA)was used as carrier materials to prepare PVs by self‐assembling. And then PVs was modified by Tf and Tet‐1(Tf/Tet‐1‐PVs). To evaluate its ac‐tive targeting, coumarin‐6 was used as a fluorescent probe to analyze cellular uptake of PVs for both BCEC and Neuro‐2a cells. Results PVs was about 80 nm with rounded shape and had obvious film structure. Tf/Tet‐1‐PVs exhibited a significant role in promoting cellular uptake for both BCEC and Neuro‐2a cells compared with control and single ligand‐modified group. Conclusion PVs modified with dual ligands could promote the cell uptake for both brain capillary cells and nerve cells.