Rapid identification of chemical components in Artemisiae argyi folium by RRLC-TOFMS
10.3969/j.issn.1006-0111.2014.06.012
- VernacularTitle:应用RRLC-TOFMS技术快速鉴别中药艾叶中的化学成分
- Author:
Ling LI
1
;
Lei L
;
Xin DONG
;
Diya LV
;
Ziyang LOU
Author Information
1. 第二军医大学药学院分析测试中心
- Keywords:
RRLC;
Artemisiae argyi folium;
chemical components;
TOFMS
- From:
Journal of Pharmaceutical Practice
2014;(6):448-452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the chemical components in Artemisiae argyi folium by rapid resolution liquid chromatog-raphy-time of flight mass spectrometry (RRLC-TOFMS).Methods The separation was performed on an Agilent Eclipse C18 column (2.1 mm ×100 mm,1.8 μm ).The mobile phase consisted of acetonitrile (A) and 0.1% formic acid (B) were in gradient elu-tion.The flowing rate was 0.35 ml/min, the injection volume was 1μl and the temperature of column was 40℃.Time of flight mass spectrometer ( TOFMS) with electro spray ion source ( ESI) was applied to qualitative analysis under the positive ion mode, and mass scan range was m/z 100-1 500 .Results 31 chemical compounds in Artemisiae argyi folium were identified unequivocally .Conclu-sion A rapid and efficient RRLC-TOFMS approach for identifying the chemical constituents of Artemisiae argyi folium had been suc-cessfully established,which paved a way for quality control and further in vivo studies of Artemisiae argyi folium.
