Study on local pharmacokinetics of baicalin gel after transdermal administration in rats by skin microdialysis in vivo
10.3969/j.issn.1006-0111.2014.06.011
- VernacularTitle:用在体皮肤微透析法研究黄芩苷凝胶经大鼠皮肤吸收的局部药动学
- Author:
Yuefen LOU
1
;
Quangang ZHU
;
Benming YOU
;
Jie LI
;
Xiaoyan GU
;
Ruoxi ZHANG
Author Information
1. 上海市第一人民医院分院药剂科
- Keywords:
baicalin gel;
microdialysis;
dermatopharmacokinetics;
rat
- From:
Journal of Pharmaceutical Practice
2014;(6):444-447
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a novel skin microdialysis technology in vivo,and to determine the pharmacokinetic of ba-icalin after transdermal administration in rats. Methods An HPLC-MS/MS method used for the determination of baicalin in skin mi-crodialysis samples was established ,SD rats were pretreated with skin microdialysis operation under anesthesia , and then the baicalin gel was applied to the skin surface of probe in vivo.The baicalin concentration of skin microdialysates was determined , the time curve of baicalin concentration was drawn and the topical pharmacokinetics parameters of percutaneous absorption was calculated. Results Baicalin was optimized at the transitions m/z 447.3→271.2.The linearity correlation was good and the assay exhibited good precision and accuracy.The subcutaneous probe recovery of baicalin in vivo was(24.40 ±0.91)%and was stable over the 240 min study peri-od.Baicalin could be detected in the microdialysis samples after transdermal administration , and its concentration continued to rise in 8 h.AUC0-t in skin tissue was(50.04 ±34.17) mg· min· L-1. Conclusion The method of skin microdialysis in vivo could be used in the local pharmacokinetic research of baicalin.
