Protein stability and hemolytic activity of tentacle extract from the jellyfish Cya-nea capillata
10.3969/j.issn.1006-0111.2014.06.009
- VernacularTitle:发形霞水母触手提取物的蛋白稳定性及其溶血活性
- Author:
Yinlong CHANG
1
;
Liang XIAO
;
Jiemin ZHENG
;
Qianqian WANG
;
Liming ZHANG
Author Information
1. 第二军医大学海医系海洋生物技术教研室
- Keywords:
Cyanea capillata;
tentacle extract;
stability;
hemolytic activity
- From:
Journal of Pharmaceutical Practice
2014;(6):434-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influencing factors of the protein stability and hemolytic activity of tentacle extract ( TE) from the jellyfish Cyanea capillata .Methods Effects of various factors and treatments on the protein stability and hemolytic ac-tivity of TE were explored by protein detection , hemolytic assay and SDS-PAGE analysis.Results TE caused a significant and dose-dependent hemolytic effect , and the HU50 of TE against 0.5%erythrocyte suspensions from SD rats was 226μg/ml.A 40℃water bath for 1 hour could effectively remove the contaminating proteins in TE .TE retained hemolytic activity at 4℃for 28 days but it was unsta-ble when kept at 25℃over 3 days.TE was active in the range from pH 6.0 to 11.0 and the optimum pH was 8.0.Various buffer solu-tions had significantly different effects on the stability and hemolytic activity of TE , and a good salting-out effect was observed on the hemolytic protein of TE while the concentration of ammonium sulfate solutions was greater than 26%.Conclusion A 40℃water bath for 1 hour could effectively remove the contaminating proteins in TE and reduce its viscosity .The optimum conditions for maintaining stability and hemolytic activity of TE were 4℃ and pH 8.0.The salting-out effect from 26% and more ammonium sulfate solutions would be conducive to the enrichment of hemolytic protein .
