Validation of method for microbial limit test of fast vertical liquid hand disinfect-ant gel

VernacularTitle:快立洁手消毒凝胶的微生物限度检查方法学验证
Author: Qiaoting TAN ¹ ; Jia YAN ; Xin ZHOU ; Hongtao SONG
Author Information

1. 南京军区福州总医院药学科
Keywords: fast vertical liquid hand disinfectant gel; microbial limit test; method validation; membrane filtration method
From: Journal of Pharmaceutical Practice 2014;(5):366-367,382
CountryChina
Language:Chinese
Abstract: Objective To establish a method for microbial limit test of fast vertical liquid hand disinfectant gel .Methods The membrane filtration method, in accordance with the Chinese Pharmacopoeia 2010 Edition (part two) relevant requirements of microbial limit test method for validation of microbial limit test for fast vertical liquid hand disinfectant gel .Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Candida a lbicans, Aspergillus niger were taken as test bacteria , Pseudomonas aeruginosa and Staphylococcus aureus were taken as control bacteria .Verification test was carried out in parallel test for 3 independent , recoveries and every trail rate were calculat-ed.Results In bacteria, molds and yeasts count method validation test , recovery test strain rate , the control bacteria test results met the requirements.Conclusion The membrane filtration method was feasible and effective , which could be used for microbial limit test of fast vertical liquid hand disinfectant gel .