Study on synthesis and cyto-toxicity of polyethylene glycol-b-polylysine as poten-cial miRNA carrier

VernacularTitle:微小RNA传递载体聚乙二醇-b-聚赖氨酸的合成及细胞毒性研究
Author: Haifeng REN ¹ ; Yingkui ZHAO ; Feng YANG ; Yuan YU ; Zhiqiang MA
Author Information

1. 第二军医大学药学院
Keywords: non-viral vector; polyethylenimine; miRNA; cyto-toxicity
From: Journal of Pharmaceutical Practice 2014;(5):362-365,373
CountryChina
Language:Chinese
Abstract: Objective To synthesize miRNA carrier PEG-b-PLL and to testify the stability , encapsulation efficiency and cyto-toxicity of its complexes .Methods H1 NMR was used to determine the degree of polymerization of PLL , 4%agarose gel electrophore-sis was used to determine entrapment of the polyer to the miRNA;then dynamic light scattering ( DLS) was used to measure the hydro-dynamic parameter such as size , polydispersity index ( PDI) and zeta potential of the polyplexes .The entrapment efficiency was deter-mined by ultraviolet-visible spectrophotometer , and finally , the cyto-toxicity of PEG-b-PLL was evaluated by CKK-8 kit with K562 cell lines.Results The characteristics indicated polyplexes prepared by PEG-b-PLL and miRNA fulfill the demand of being the gene carri-er of miRNA because of low cyto-toxicity , high encapsulation efficiency and stability .Conclusion The miRNA carrier PEG-b-PLL had good character and low cyto-toxicity.It showed considerable potential as an efficient miRNA carrier .