Determination of febuxostate in human plasma by LC-MS-MS

VernacularTitle:LC-MS-MS法测定人血浆中非布司他浓度
Author: Nan JIANG ¹ ; Yongge YANG ; Lixue SONG ; Xueting XU
Author Information

1. 北京军区总医院药理科
Keywords: febuxostate; LC-MS-MS; plasma concentration
From: Journal of Pharmaceutical Practice 2014;(5):354-356
CountryChina
Language:Chinese
Abstract: Objective To establish a LC-MS-MS method for determining febuxostate in human plasma .Methods Febuxostate added into blank plasma was sedimented by acetonitrile , and the supernatant was determined by LC-MS-MS.Analytical column was Thermo Biobasic-8,5 μm,50 mm ×2.1 mm(ID).The mobile phase consisted of acetonitrile-10 mmol/L ammonium acetate (0.05%acid=70:30 at a flow rate of 0.2 ml/min.Mass spectrum conditions:ESI-was performed in the SRM mode using target ions m/z 315→271 (10 eV)(febuxostate), m/z 360→274 (18 eV)(bezafibrate), SP 3 500 kV, SGP 10 Arb, AGP45 Arb, TEM 270℃.Results The calibration curve was linear over the range of 10-8 000 μg/L.The LLOQ of Febuxostate in plasma was 10 μg/L.The extracted recovery was >85%.The intra-and inter-day RSD were <15%.Conclusion The method was sensitive , simple and accurate to deter-minate febuxostate plasma concentration and to study pharmacokinetics of febuxostate .