Determination of the polysaccharide in Verdihong paint
10.3969/j.issn.1006-0111.2014.05.009
- VernacularTitle:蒽酮染色法测定维地红涂剂中多糖的含量
- Author:
Xueying YU
1
;
Mingquan CHANG
;
Fang CHEN
;
Gang WANG
Author Information
1. 湖北医药学院附属太和医院
- Keywords:
Verdihong paint;
polysaccharide;
content determination
- From:
Journal of Pharmaceutical Practice
2014;(5):352-353,356
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the determination method of the polysaccharide in Verdihong paint .Methods The anhy-drous dextrose was used as the control , 0.2%anthrone-sulfuric acid as the stain , the content of polysaccharide was determined by ul-traviolet spectrophotometer at the wavelength of 620 nm.Results The polysaccharide curve was linear within a range of 1.9~34.2μg/ml, linear equation was Y=0.033 X-0.006 7 (r=0.999 9), the average recovery was 98.6% with RSD of 1.06%(n=6). Conclusion This method was convenient , stable and coud be used to assay content of the polysaccharide in Verdihong paint .
