Screening of active compounds of pro-angiogenic in Shexiang Baoxin pill

VernacularTitle:麝香保心丸促进血管新生作用的活性成分筛选
Author: Chao LV ¹ ; Huimei HUANG ; Wanlin CHANG ; Runhui LIU
Author Information

1. 福建中医药大学药学院
Keywords: Shexiang Baoxin pill (SBP); ginsenosideRg3; ginsenosideRh2; angiogenesis
From: Journal of Pharmaceutical Practice 2014;(5):344-347,351
CountryChina
Language:Chinese
Abstract: Objective To screen and identify the main pro-angiogenic compounds of Shexiang Baoxin pill ( SBP) presenting in the plasma.Methods The pro-angiogenic effects of SBP and its compounds absorbed into blood were measured by the cell prolif-eration and cell migration assays by xCELLigence .And the cell tube formation and rat aortic ring models were established to evaluate their pro-angiogenic effect.Results SBP(10 -4 ~10 -2 μg/ml), ginsenoside Rg3(1 ~10 μmol/L) and ginsenoside Rh2(1 ~10μmol/L)significantly stimulated human umbilical vein endothelial cells (HUVECs) proliferation, migration and tube-like structures formation at different concentrations (P<0.05).In addition, compared to the control group, only the high concentration group of SBP (10 -2 μg/ml), Rg3(10 μmol/L) and Rh2(10 μmol/L)could induce endothelial cell sprouting from the aortic ring(P<0.05) .Conclusion SBP, ginsenosideRg3 and Rh2 exhibited significantly pro-angiogenic effect in vitro.