Determination of content of cucurbitacin B in cucurbitacins by HPLC
10.3969/j.issn.1006-0111.2014.04.015
- VernacularTitle:应用高效液相色谱法测定葫芦素片中葫芦素B的含量
- Author:
Jiatai DOU
1
;
Shengwen LI
Author Information
1. 解放军209医院药械科
- Keywords:
HPLC;
cucurbitacin tablet;
cucurbitacin B
- From:
Journal of Pharmaceutical Practice
2014;(4):294-296
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determina the content of cucurbitacin B in cucurbitacins .Methods The highly effective liquid phase chromatography was used,with Waters Symmetry C18(250 mm ×4.6 mm 5μm) column.The mobile phase was acetonitrile-wa-ter (51:49), the flow rate was 1 ml/min, column temperature was 25 ℃, the detection wavelength was 228 nm.Results Cucurb-itacin B regression equation:Y=1 067.3C-0.508 4 (r=0.999 9), the average recovery rate was 99.39%(n=6), RSD was 0.56%, which showed that this method had a good recovery rate .Conclusion The HPLC method for the determination of content of cucurbitacin B in cucurbitacins was simple , reproducible , accurate .
