Screening of main active components against cardiomyocyte hypoxia/reoxygen-ation injury in shexiang baoxin Pill
10.3969/j.issn.1006-0111.2014.03.012
- VernacularTitle:麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选
- Author:
Lin HAN
1
;
Chao LV
;
Min LI
;
Huimei HUANG
;
Wanlin CHANG
;
Chengcheng PENG
;
Runhui LIU
Author Information
1. 第二军医大学药学院
- Keywords:
Shexiang Baoxin Pill ( SBP);
cardiomyocyte;
hypoxia/reoxygenation model;
screening of active components
- From:
Journal of Pharmaceutical Practice
2014;(3):209-211,219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill ( SBP) absorbed in blood against hypoxia/reoxygenation injury .Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats (1~3 days old) and were used to build hypoxia/reoxygenation injury model.The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method.Results SBP showed significant protective effect against cardiomyocytes hypoxia /reoxygenation injury atthe concentration of 50 μg/ml.Ginsen-oside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect a -gainst cardiomyocytes hypoxia/reoxygenation injury .Conclusion SBP have the protective activity against cardiomyocytes hypoxia /reoxygenation injury , and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP .This experiment offered basis for further pharmacodynamics and mechanism study of SBP .
