Antidiarrheal potential of Distemonanthus benthamianus Baillon. extracts via inhibiting voltage-dependent calcium channels and cholinergic receptors
- Author:
Nana Yousseu WILLIAM
1
;
Ateufack GILBERT
;
Mbiantcha MARIUS
;
Khan SHAMIM
;
Rasheed Majid HAFIZ
;
Atsamo ALBERT
;
Shah Jabbar ABDUL
;
Kamanyi ALBERT
;
Khan TAOUS
Author Information
- Keywords: Distemonanthus benthamianus; HPLC; Calcium-channels blocking; Anticholinergic
- From:Asian Pacific Journal of Tropical Biomedicine 2019;9(11):449-455
- CountryChina
- Language:Chinese
- Abstract: Objective: To evaluate spasmolytic mechanisms of aqueous and methanolic extracts from Distemonanthus benthamianus trunk-bark. Methods: Spasmolytic activities of extracts were evaluated in vitro on spontaneous and potassium chloride-induced jejunum contractions, or against cholinergic [acetylcholine (0.3μmol/L)] stimulations. High performance liquid chromatography analysis of both extracts was performed in reference to standard compounds. Results: Extracts developed concentration-dependent inhibitory activities. The methanolic extract, which revealed better activity, produced spasmolytic and myorelaxant effects at concentrations of 0.01-0.30 mg/mL with EC50 of 0.06 and 0.09 mg/mL (95％ CI: 0.03-0.3 mg/mL), respectively. Its anticholinergic effect was obtained at the same concentrations with EC50 of 0.11 mg/mL (95％ CI:0.03-0.3 mg/mL). Chromatograms showed the presence of gallic acid in both extracts, rutin being only detected in the aqueous extract. Conclusions: Distemonanthus benthamianus extracts exhibit verapamil and atropine-like activities, thus highlighting calcium channels and muscarinic receptors blocking potentials, which may be conveyed by some phenolic compounds. These results confirm the antidiarrheal activity of Distemonanthus benthamianus extracts.
