Comparison of plasma microRNA-1 and cardiac troponin T in early diagnosis of patients with acute myocardial infarction
10.5847/wjem.j.issn.1920-8642.2014.03.004
- Author:
Li-Ming LI
1
;
Wen-Bo CAI
;
Qin YE
;
Jian-Min LIU
;
Xin LI
;
Xiao-Xing LIAO
Author Information
1. Department of Emergency Medicine
- Keywords:
MicroRNA-1;
High sensitive cardiac troponin T;
Acute myocardial infarction;
Biomarker;
Early diagnosis;
Specificity;
Sensitivity
- From:
World Journal of Emergency Medicine
2014;5(3):182-186
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Early reperfusion can effectively treat acute myocardial infarction (AMI) and reduce the mortality significantly. This study aimed to compare the role of plasma microRNA-1 (miR-1) and cardiac troponin T (cTnT) in early diagnosis of AMI patients. METHODS: From May 2011 to May 2012, plasma samples were collected from 56 AMI patients and 28 non-AMI controls. The expression of plasma miR-1 was measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and the level of plasma cTnT was measured using electrochemiluminescence-based methods on an Elecsys 2010 Immunoassay Analyzer. SPSS 16.0 was used for the statistical analysis of the results. Data were expressed as mean±standard deviation unless otherwise described. The differences about clinical characteristics between the AMI patients and controls were tested using Student'st test or Fisher's exact test. The Mann-WhitneyU test was conducted to compare the expression of microRNAs between the AMI patients and controls. MicroRNAs expression between different intervals of the AMI patients was compared using Wilcoxon's signed-rank test. The receiver operating characteristic (ROC) curve was established to discriminate the AMI patients from the controls. RESULTS: In the present study, the expression of plasma miR-1 was significantly increased in the AMI patients compared with the healthy controls (P<0.01). The plasma miR-1 in the AMI patients decreased to the normal level at 14 days (P>0.05). The expression of plasma miR-1 was not related to the clinical characteristics of the study population (P>0.05). ROC curve analyses demonstrated that miR-1 was specific and sensitive for the early diagnosis of AMI, but not superior to cTnT. CONCLUSION: Plasma miR-1 could be used in the early diagnosis of AMI, but it is similar to cTnT.
