Lab-on-chip for the detection of food borne pathogenic bacteria
- VernacularTitle:微流体芯片技术检测食源性致病菌的应用研究
- Author:
Jiong CHEN
1
;
Li-Hong TU
;
Yan-Xin CHEN
;
Sheng-Ying QIN
Author Information
1. 上海市疾病预防控制中心
- Keywords:
Lab-on-chip;
Food borne pathogen;
Detection method
- From:
Shanghai Journal of Preventive Medicine
2014;(6):330-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up an onsite protocol for abrupt emergencies of public health e-vents, with both the sensitivity and accuracy of the lab-on-chip(microfluidic chip), for the detection of food borne pathogenic bacterium including Vibrio cholera , Salmonella , Vibrio parahaemolyticus and Shigellaare exanimated . [ Methods] By comparison of the results from the chips and fluorescence quantitative PCR were analyzed the specificity , sensitivity, accuracy and repeatability of the Lab-on-chip. [ Results] Acceptable specificity , accuracy and repeatability of the chips had been well achieved , the detection limit of the chips for the Vibrio cholera , Salmonella, Vibrio parahaemolyticus and Shigella was 5 ×103 ~103 DNA Copies/mL. [ Conclusion] Lab-on-chip is believed to be a fast , convenient , efficient tool with accept-able accuracy for public health emergencies .
