Simultaneous determination of three compounds in penthorum Chinense pursh by HPLC
10.3760/cma.j.issn.1673-4246.2019.08.016
- VernacularTitle:HPLC法同时测定赶黄草中槲皮素、山柰酚及乔松素含量
- Author:
Zhen KANG
1
;
Chundi LIU
;
Xiaoyan LIU
;
Zhitao JIANG
;
Xingyue FENG
;
Yongmei ZHANG
Author Information
1. 南京中医药大学药学院药学院 210023
- Keywords:
Penthorum Chinense pursh;
Quercetin;
Kaempferol;
Pinocembrin;
High performance liquid chromatography
- From:
International Journal of Traditional Chinese Medicine
2019;41(8):863-866
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the simultaneous content determination ofquercetin,kaempferol and pinocembrin in Penthorum Chinense Pursh.Methods The HPLC analysis was carried out on Alltima C18 (250 mm × 4.6 mm,5 μm) with a mixture of methanol-0.2％ phosphate (45:55) as the mobile phase.The determination wavelength was set at 270 nm with the flow rate of 1.0 ml/min.The column temperature was set at 30 ℃.Results The quercetin,kaempferol and pinocembrin showed good linearity in the range of 0.316-10.120 μg (r=0.999 9),0.012-0.397 μg (r=0.999 6) and 0.063-2.016 μg (r=0.999 8) respectively.The average recovery rates of quercetin,kaempferol and pinocembrin were 99.34％ (RSD=1.51％),99.76％ (RSD=l.96％) and 98.34％ (RSD=1.56％) respectively.Conclusions The method is accurate and sensitive,which can be used for the content determination of quercetin,kaempferol and pinocembrin in Penthorum Chinense Pursh.
