Mechanism of Ermiao power in the treatment of rheumatoid arthritis based on urine metabolomics technology
10.3760/cma.j.issn.1673-4246.2019.08.014
- VernacularTitle:基于尿液代谢组学技术的二妙散抗类风湿关节炎作用机制研究
- Author:
Hongdan XU
1
;
Bo ZHANG
;
Yuan YAO
;
Mingjie LI
;
Guoliang LIU
;
Xiaohong DONG
;
Bin LIU
Author Information
1. 黑龙江中医药大学佳木斯学院 154007
- Keywords:
Er Miao San;
Arthritis,rheumatoid;
Metabolomics;
Linoleic acid;
Molecular mechanisms of pharmacological action (TCD);
Rats
- From:
International Journal of Traditional Chinese Medicine
2019;41(8):852-857
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti-rheumatoid arthritis mechanism of Ermiao powder by high-throughput urine metabolomics.Methods The rats were randomly divided into control group,model group and administration group with 8 rats in each group.The rat model of rheumatoid arthritis was established by intradermal injection of complete Freund's adjuvant.Rats in control group were given Ermiao power solution 0.108 g/ml by gavage.The degree of joint swelling in rats was observed and scored.On this basis,metabolic data of rat urine samples were collected for metabolomic analysis.Unsupervised and supervised pattern recognition technology was used to analyze the high-throughput biological information data and reduce the dimension.Metabolic information related to rheumatoid arthritis was screened and focused to clarify the pathogenesis of rheumatoid arthritis and the therapeutic mechanism of Ermiao power.Results Compared with the model group,the swelling degree of the foot (1.93 ± 0.11 vs.2.36 ± 0.19) in Ermiao power group significantly decreased (P＜0.01).Metabolic profiles showed that the metabolic distribution of healthy rats was significantly separated from that of model rats,and the treatment group was in the middle of the two groups.From the macro-metabolic point of view,the metabolism of model rats changed dramatically.The Ermiao power had a good intervention effect on rheumatoid arthritis.Thirteen biomarkers related to rheumatoid arthritis were identified by database matching,including linolenic acid,arachidonic acid,5,6-EET,alpha-lactose,sucrose,trehalose,prostaglandins and leukotriene C4.It involved linoleic acid metabolism,arachidonic acid metabolism,starch and galactose metabolism and sphingolipid metabolism.Conclusions The Ermiao power has significant therapeutic effect on rheumatoid arthritis rats.Regulation of the linoleic acid metabolism,arachidonic acid metabolism,starch and galactose metabolism may be the mechanism of its treatment of rheumatoid arthritis.
